Phgwfs7.0

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August undefined, 2024

Web20. okt 2016 · The vector pH35 was constructed from Gateway promoter-reporter vector pHGWFS7, which contained a GFP-GUS fusion for plant expression, hygromycin … Web1. sep 2024 · Phylogenetic analysis was performed using MEGA 5.0 as described by [35]. The knockout vector was designed according to a CRISPR/Cas9 System for targeting …

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Web14. máj 2014 · Columbia (Col-0) background ecotype and mutant seeds were stratified on M3 compost for 72 hours in the dark at 4°C before transfer to a growth room with 10 … Web2. aug 2024 · winwin7为大家带来的【SnapGene】自带破解文件和激活工具，亲测可完美破解激活软件！ SnapGene 中文破解版安装激活 1，双击SnapGene .exe，安装软件 2，软 … campbell biology 12th edition online

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Web23. júl 2010 · The glucose-6-phosphate/phosphate antiporter GPT1 is a major route of entry of carbon into non-photosynthetic plastids. To discover its importance in oilseeds, we … WebpHGWFS7,0 13090 bp Egfp gus ccdB Sm/SpR Hyg T35S attR1 attR2 LB RB pDONR PGI1 promoter 3776 bp Zeo (R) attL1 attL2 PGI1 promoter EM7 promoter ... Treatment 1 … WebHello, I am using pBGWFS7.0 Vector as destination vector in LR reaction. I could not find this Vector sequence on net, Vector map is available on net. Can somebody tell me the … campbell biology 6th edition pdf

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Webwere sterilized with 0.3% Sodium Hypochlorite and pre-germinated on 0.9% bactoagar plates at 30 C. Seven day old seedlings were transferred to sterilized sand and grown for two … WebStrains materials and growth conditions. Arabidopsis thaliana (ecotype Col-0) and atsuc4 mutants were grown in a growth chamber in soil under long-day (LD) conditions (16 h … first spear cummerbundWebArabidopsis thaliana ecotype Columbia-0 was used as a source of wild-type plant material. Seeds were surface-sterilized and cul- ... for protein subcellular localization (pH7FWG2,0), … campbell biology 12th ppt

"Web24. nov 2024 · The athsp22.0–1 represent the T-DNA insertion site in the promoter region and athsp22.0–2 in 5′ UTR region Full size image The T-DNA insertion lines were localized … " - Phgwfs7.0

Phgwfs7.0

http://www.winwin7.com/soft/46492.html WebThe destination vector is pHGWFS7,0 (13 kb). The donor vector is pDONR221. I attached reference protocols for single-step BP/LR gateway. Any comment and advice will be really …

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Web0 votes 0 thanks. James W. Borrone. You state that you sent your insert off for sequencing. It would be best to contact whomever you sent the insert to, and discuss it with them. 0 votes 0 thanks. Daniel M Cohen. This is a very confusing post. Can you please share what vector(s) you are using and what time of sequencing oligos (designed against ... WebpHGWFS7.0植物启动子报告载体，带有EGFP绿色荧光报告基因和GUS报告基因，Sp壮观霉素抗性，hyg潮霉素植物筛选标记。 gateway技术插入检测启动子。 BioVector NTCC质粒载 …

Webantiporter genes into the GATEWAYTM promoter cloning vector pHGWFS7. The Pokkali promoter expressed the β-glucuronidase or GUS gene ~25-fold more efficiently than the … WebFor the pRAPTOR1B::GUS:eGFP transcriptional fusion, 1,360 bp upstream of the of RAPTOR1B (AT3G08850) were amplified by PCR, cloned into the pDONR221TM P1P2 by BP reaction (BP clonase, Thermofisher), and sub-cloned into the destination vector pHGWFS7.0 by LR reaction (LR clonase, Thermofisher).

WebThe constructed destination vector pHGWFS7.0_Adh was transformed into LBA4404 strain of Agrobacterium by electroporation. The transformed plate was kept at 28°C for 72 hrs … WebCloning and Characterization of Alcohol Dehydrogenase (Adh) Promoter Region for Expression Under Submergence and Salinity Stress

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Web27. máj 2014 · Europe PMC is an archive of life sciences journal literature. campbell biology 3rd canadian edition pdfWebVIB-UGent Center for Plant Systems Biology. Gateway Vectors. Collection. pKGWFS7. campbell biology a global approachWeb28. feb 2024 · In cold-primed plants, the pleiotropically stress regulated genes CHS (chalcone synthase; At5g13930) and PAL1 (phenylalanine ammonium lyase; At2g37040) … first spear backpackWeb1. aug 2010 · The original vector for promoter analysis, pHGWFS7, employed a fused EGFP and GUS as its reporter gene and the HPT driven by the nopaline synthase promoter ( … first spear dehvad mastery pointWeb23. dec 2016 · The following binary T-DNA destination vectors were used; pMDC32 ( Curtis and Grossniklaus, 2003) and pHGWFS7.0 ( Karimi et al., 2002 ). A 1834-bp sequence corresponding to the PER57 promoter was amplified from Col-0 genomic DNA using primers listed in Supplementary Table S1. firstspear belthttp://armis.barcapps.gov.bd/home/research/detail/18006 first spear devhad gw2Web24. mar 2024 · Genomic sequences of the GFP-coding region of the pHGWFS7.0 were amplified by PCR using primer nos. 3 and 4 (Supplemental Table S1), introduced into the … campbell biology a global approach pdf