Grf–gif chimeric protein
WebOct 12, 2024 · Here, we show that expression of a fusion protein combining wheat GROWTH-REGULATING FACTOR 4 (GRF4) and its cofactor GRF-INTERACTING … WebJul 15, 2024 · GRF–GIF chimeric proteins enhance in vitro regeneration and Agrobacterium-mediated transformation efficiencies of lettuce (Lactuca spp.) 2024, Plant …
Grf–gif chimeric protein
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WebPrevious work showed that expressing a fusion of two interacting transcription factors, GROWTH-REGULATING FACTOR4 (GRF4) and GRF-INTERACTING FACTOR1 (GIF1), improved regeneration in wheat (Triticum aestivum). By overexpressing a chimeric fusion of ClGRF4 and ClGIF1, we achieved highly efficient transformation in watermelon. WebJan 12, 2024 · A GRF-GIF chimeric protein improves the regeneration efficiency of transgenic plants. Juan M Debernardi, David M Tricoli, Maria F Ercoli, Sadiye Hayta, …
WebA GRF-GIF chimeric protein improves the regeneration efficiency of transgenic plants. Debernardi JM, Tricoli DM, Ercoli MF, Hayta S, Ronald P, Palatnik JF, Dubcovsky J. Nat … WebDec 15, 2024 · A GRF–GIF chimeric protein improves the regeneration efficiency of transgenic plants. Nat. Biotechnol. 38, 1274–1279 (2024). Article CAS Google Scholar ...
WebA red fluorescent protein-encoding gene was added to both constructs to enable rapid quantification of transgenic regeneration rates. The chimeric GRF-GIF sequences were cloned into an expression vector and poplar as well as eucalypt tissue explants were transformed using Agrobacterium. WebAug 24, 2024 · Here, we show that expression of a chimeric protein including wheat GROWTH-REGULATING FACTOR 4 (GRF4) and its cofactor GRF-INTERACTING FACTOR 1 (GIF1) dramatically increases the efficiency...
WebApr 14, 2024 · Then, the miR396-GRF-GIF-SWI/SNF module played an essential role in GA signaling to control plant growth . In addition, the miR166e/calmodulin-like protein module was newly identified in our research, and the miR166 has been revealed to be involved in salt stress response and GA biosynthesis and catabolism .
WebA GRF-GIF chimeric protein improves the regeneration efficiency of transgenic plants. Debernardi JM, Tricoli DM, Ercoli MF, Hayta S, Ronald P, Palatnik JF, Dubcovsky J Nat Biotechnol. 2024 Oct 12. pii: 10.1038/s41587-020-0703-0. doi: 10.1038/s41587-020-0703-0. PubMed Article Plasmids from Article. ID Plasmid Purpose ... cecily oakleyWebDec 5, 2024 · The study was based on 2100 backcross progenies derived from crosses of fertility restorers with 9 European winter wheat lines. BC1F6 progenies were evaluated at PAU, Ludhiana in 2024-18 for key... buttering up crosswordWebJan 14, 2024 · Furthermore, Growth-Regulating Factor 4 (GRF4) and its cofactor GRF-Interacting Factor 1 (GIF1) have been recently shown to increase the transformation frequencies in both monocots and dicots, most likely by regulating the cell proliferation and in the transition between stem cells to transit-amplifying cells. cecily nicholson interesting factscecily nicole cromartie of scotland countyWebJan 25, 2024 · GRF-GIF chimeric proteins from multiple source species enhance in vitro regeneration in both wild and cultivated lettuce. In addition, they enhance regeneration in multiple types of lettuce including butterheads, romaines, and crispheads. The ability of plants to regenerate in vitro has been exploited for use in tissue culture systems for plant ... buttering the muffinWebOct 12, 2024 · In line with this finding, researchers at UC Davis concurrently observed that overexpression of a chimeric protein containing GRF and its transcriptional co-activator GIF greatly increased the transformation efficiency in … cecily nowell-smithWebBased on the observation that GRFs and GIFs interact to form a protein complex 15, we decided to evaluate the effect of a GRF-GIF chimeric protein. Here we show that … buttering trowel